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NONO Antibody / p54nrb Antibody

Non-POU domain-containing octamer-binding protein is a protein that in humans is encoded by the NONO gene. This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, ICC (FFPE) : 1-2 µg/mL, FACS: 1-3ug/10^6 cells

UniProt

Q15233

Host

Mouse

Reactivity

Human

Immunogen

Amino acids MQSNKTFNLEKQNHTPRKHHQHHHQQQHHQQQQQQ were used as the immunogen for the NONO antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

11E2-

Applications

WB, ICC, FACS

Purity

Protein G affinity

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the NONO antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NONO antibody is available for research use only.

Storage Conditions

After reconstitution, the NONO antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NONO antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) A549, 5) SW620, 6) PANC-1, 7) U-2 OS and 8) K562 lysate with NONO antibody at 0.5ug/ml. Predicted molecular weight ~54 kDa.

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