TRX Antibody / Thioredoxin / TXN

Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunocytochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (mouse recombinant protein), Immunofluorescence: 2-4 µg/mL

UniProt

P10639

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids V2-A105 from the mouse protein were used as the immunogen for the TRX antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC, Direct ELISA, IF

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the TRX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TRX antibody is available for research use only.

Storage Conditions

After reconstitution, the TRX antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TRX antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

Western blot testing of mouse 1) testis, 2) kidney, 3) small intestine, 4) spleen, 5) stomach, 6) lung, 7) smooth muscle and 8) heart lysate with TXN antibody at 0.5ug/ml. Predicted molecular weight ~12 kDa.