NOX5 Antibody

NOX5 (Nadph Oxidase 5) is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. It is found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, NOX5 was identified as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells (HC), but not normal B cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)

UniProt

Q96PH1

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids E13-F197 from the human protein were used as the immunogen for the NOX5 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF/ICC, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the NOX5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Storage Conditions

After reconstitution, the NOX5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NOX5 antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) HeLa, 2) placenta, 3) SCG-7901, 4) Jurkat and 5) THP-1 lysate with NOX5 antibody at 0.5ug/ml. Predicted molecular weight: ~86 kDa.