LUM Antibody / Lumican

Lumican, also known as LUM, is an extracellular matrix protein that, in humans, is encoded by the LUM gene on chromosome 12. Lumican is a major keratan sulfate proteoglycan of the cornea but is ubiquitously distributed in most mesenchymal tissues throughout the body. And Lumican is involved in collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. Corneal transparency is possible due to the exact alignment of collagen fibers by lumican (and keratocan) in the intrafibrillar space.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (recombinant human protein)

UniProt

P51884

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids A50-N338 from the human protein were used as the immunogen for the LUM antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the LUM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This LUM antibody is available for research use only.

Storage Conditions

After reconstitution, the LUM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the LUM antibody should be determined by the researcher.

Location

Secreted, cytoplasmic

Image Legend

Western blot testing of human 1) placenta, 2) Caco-2, 3) CCRF-CEM, 4) HeLa and 5) Jurkat lysate with LUM antibody at 0.5ug/ml. Expected moleculer weight: ~40 kDa (unmodified), ~60 kDa (glycosylated) .