LMAN1 Antibody / ERGIC-53

Protein ERGIC-53, also known as ER-Golgi intermediate compartment 53 kDa protein or lectin mannose-binding 1, is a protein that in humans is encoded by the LMAN1 gene. It is mapped to 18q21.32. The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)

UniProt

P49257

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids K96-M458 from the human protein were used as the immunogen for the LMAN1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the LMAN1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This LMAN1 antibody is available for research use only.

Storage Conditions

After reconstitution, the LMAN1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the LMAN1 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) human A431, 2) human HepG2, 3) rat liver, 4) rat heart, 5) rat C6, 6) mouse liver, 7) mouse heart and 8) mouse NIH 3T3 cell lysate with LMAN1 antibody. Predicted molecular weight ~53 kDa.