SHIP2 Antibody / INPPL1

SH2-domain containing Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2 is an enzyme that in humans is encoded by the INPPL1 gene. The protein encoded by this gene is an SH2-containing 5'-inositol phosphatase that is involved in the regulation of insulin function. The encoded protein also plays a role in the regulation of epidermal growth factor receptor turnover and actin remodelling. Additionally, this gene supports metastatic growth in breast cancer and is a valuable biomarker for breast cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Immunofluorescence/Immunocytochemistry (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)

UniProt

O15357

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids R1172-K1258 from the human protein were used as the immunogen for the SHIP2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/ICC, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the SHIP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SHIP2 antibody is available for research use only.

Storage Conditions

After reconstitution, the SHIP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SHIP2 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of human 1) placenta, 2) HeLa, 3) U-2 OS, 4) PC-3, 5) Caco-2, 6) A549 and 7) K562 lysate with SHIP2 antibody at 0.5ug/ml. Predicted molecular weight ~139 kDa (isoform 1), ~113 kDa (isoform 2) .