GOLPH3 Antibody

Golgi phosphoprotein 3 (GOLPH3) is a human protein encoded by GOLPH3 gene. It is mapped to 5p13.3. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (recombinant human protein)

UniProt

Q9H4A6

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids K56-K298 from the human protein were used as the immunogen for the GOLPH3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the GOLPH3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This GOLPH3 antibody is available for research use only.

Storage Conditions

After reconstitution, the GOLPH3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the GOLPH3 antibody should be determined by the researcher.

Location

Cytoplasm, plasma membrane

Image Legend

Western blot testing of human 1) HeLa, 2) placenta, 3) U-2 OS, 4) Caco-2, 5) SW620 and 6) A549 lysate with GOLPH3 antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa.