EML4 Antibody / Echinoderm microtubule-associated protein-like 4

Echinoderm microtubule-associated protein-like 4 is a protein that in humans is encoded by the EML4 gene. This gene is a member of the echinoderm microtubule associated protein-like family, and it mapped to 2p21. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL (recombinant human protein)

UniProt

Q9HC35

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids K207-N423 from the human protein were used as the immunogen for the EML4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the EML4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This EML4 antibody is available for research use only.

Storage Conditions

After reconstitution, the EML4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the EML4 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human ovarian cancer tissue with EML4 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.