Mcp2 Antibody / Ccl8

CCL8, also known as monocyte chemoattractant protein 2 (MCP2), is a protein that in humans is encoded by the CCL8 gene. It is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. MCP-2 is chemotactic for and activates many different immune cells, including mast cells, eosinophils and basophils, (that are implicated in allergic responses), and monocytes, T cells, and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to several different cell surface receptors called chemokine receptors. These receptors include CCR1, CCR2B, CCR3 and CCR5.

Product Specifications

CAS Number

9007-83-4

Specifications

IHC (FFPE) : 1-2 µg/mL, ELISA (Capture) : 1-5 µg/mL (recombinant mouse protein)

UniProt

Q9Z121

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids G24-P97 from the mouse protein were used as the immunogen for the Mcp2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, ELISA (Capture)

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Mcp2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Mcp2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Mcp2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Mcp2 antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC staining of FFPE mouse spleen with Mcp2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.