Amphiregulin Antibody

Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor (EGF) family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha) . This protein interacts with the Epidermal growth factor receptor (EGFR) to promote the growth of normal epithelial cells. It is mapped to 9q32. It has been shown to play a role in immunity, inflammation, tissue repair, and lung and mammary gland development. Homozygous knockout mice for this gene exhibit impaired immune system regulation in the skin and gene expression changes characteristic of chronic liver damage.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA (capture) : 1-5 µg/mL (recombinant mouse protein)

UniProt

P31955

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids V100-K191 from the mouse protein were used as the immunogen for the Amphiregulin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Amphiregulin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Amphiregulin antibody is available for research use only.

Storage Conditions

After reconstitution, the Amphiregulin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Amphiregulin antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) rat brain and 2) mouse brain lysate with Amphiregulin antibody at 0.5ug/ml. Molecular weight: 28 kDa (non-glycosylated), ~50 kDa (glycosylated pro form), ~43 kDa (predominant glycosylated soluble form) as well as other, smaller, soluble and membrane bound forms.