Alpha 1 microglobulin Antibody / AMBP / Bikunin

Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)

UniProt

P02760

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids G20-V203 from the human protein were used as the immunogen for the Alpha 1 microglobulin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Alpha 1 microglobulin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Alpha 1 microglobulin antibody is available for research use only.

Storage Conditions

After reconstitution, the Alpha 1 microglobulin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Alpha 1 microglobulin antibody should be determined by the researcher.

Location

Secreted

Image Legend

Western blot testing of human 1) placenta, 2) HEp-2, 3) HeLa, 4) A549 and 5) PANC-1 lysate with Alpha 1 microglobulin antibody at 0.5ug/ml. Predicted molecular weight: 26-28 kDa (Alpha 1 microglobulin), ~39 kDa (uncleaved AMBP) . These proteins may be observed at higher molecular weights due to glycosylation.