Cortactin Antibody

Cortactin, mapped to 11q13.3, is a multidomain protein. This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, IF/ICC (FFPE) : 1-2 µg/mL, FACS: 1-3ug/10^6 cells

UniProt

Q14247

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 1-105 from the human protein were used as the immunogen for this Cortactin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/ICC, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Cortactin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cortactin antibody is available for research use only.

Storage Conditions

After reconstitution, the Cortactin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Cortactin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasm, plasma membrane

Image Legend

Western blot testing of human 1) A431, 2) U-87 MG, 3) A549, 4) PC-3 and 5) HeLa lysate with Cortactin antibody at 0.5ug/ml. Predicted molecular weight ~61 kDa but routinely observed at ~80 kDa.