FLAG Tag Antibody
FLAG-Tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It represents an early example of a nanotechnology device, in that it can be attached to a protein like a molecular handle, then used to manipulate the protein, and subsequently detached. It has been used for studying proteins in living cells and for protein purification by affinity chromatography.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.1-1 µg/mL
Host
Mouse
Immunogen
Amino acids DYKDDDDK were used as the immunogen for the FLAG Tag antibody antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
1E6.
Applications
WB, ELISA, Dot blot
Purity
Protein G affinity
Format
Purified
Buffer
0.5mg/ml in PBS with 0.02% sodium azide and 50% glycerol
Reconstitution
Limitations
This FLAG Tag antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL in PBS with 0.02% sodium azide and 50% glycerol
Applications Notes
Optimal dilution of the FLAG Tag antibody should be determined by the researcher.
Image Legend
Western blot test of FLAG-tagged protein with different concentrations of FLAG tag antibody.
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