CCT3 Antibody

T-complex protein 1 subunit gamma is a protein that in humans is encoded by the CCT3 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC) . This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P49368

Host

Mouse

Reactivity

Human

Immunogen

Amino acids EPLAVKLQTYKTAVETAVLLLRIDDIVSGHKKKGDDQSRQ were used as the immunogen for the CCT3 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

12H4

Applications

Purity

Protein G affinity

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the CCT3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CCT3 antibody is available for research use only.

Storage Conditions

After reconstitution, the CCT3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CCT3 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Western blot testing of human 1) HeLa, 2) MCF7, 3) COLO-320, 4) HepG2 and 5) HT-1080 cell lysate with CCT3 antibody at 0.5ug/ml. Predicted molecular weight ~61 kDa.