XPO1 Antibody / Exportin 1 / CRM1

Exportin 1 (XPO1), also known as chromosomal maintenance 1 (CRM1), is an eukaryotic protein that mapped to human chromosome 2p16 by fluorescence in situ hybridization. This protein mediates leucine-rich nuclear export signal (NES) -dependent protein transport. It specifically inhibits the nuclear export of Rev and U snRNAs. Additionally, this protein is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. It also regulates NFAT and AP-1.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunofluorescence: 5 µg/mL

UniProt

O14980

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Human Exportin 1 recombinant protein (amino acids N966-D1071) was used as the immunogen for the XPO1 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

5G3

Applications

IHC-P, WB, FACS, IF

Purity

Protein G affinity

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the XPO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This XPO1 antibody is available for research use only.

Storage Conditions

After reconstitution, the XPO1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the XPO1 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

Western blot testing of 1) rat liver, 2) rat lung, 3) mouse liver, 4) mouse lung, 5) rabbit IgG, 6) molecular weight marker, 7) human HepG2, 8) human SMMC-7721, 9) human HeLa and 10) human Jurkat lysate with XPO1 antibody at 0.5ug/ml. Expected molecular weight ~123 kDa.