Beta Catenin Antibody

Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunocytochemistry: 2-4 µg/mL, Immunofluorescence: 2-4 µg/mL, FACS: 1-3ug/million cells

UniProt

P35222

Host

Mouse

Reactivity

Human

Immunogen

Human beta Catenin recombinant protein (amino acids A2-K233) was used as the immunogen for the Beta Catenin antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

1F6

Applications

WB, IHC-P, ICC, IF, FACS

Purity

Protein G affinity

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Beta Catenin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Beta Catenin antibody is available for research use only.

Storage Conditions

After reconstitution, the Beta Catenin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Beta Catenin antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic, cell junctions, nuclear

Image Legend

Immunofluorescent testing of FFPE human A431 cells with Beta Catenin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.