Phospho-Histone H2AX Antibody (pS139)
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation. [UniProt]
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:1000 µg/mL, IHC (FFPE) : 1:50-1:200, IF/ICC: 1:50-1:200
UniProt
P16104
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
The amino acids surrounding a phosphorylated serine at position 139 were used as the immunogen for the phospho-Histone H2AX antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
AbH41
Type
Recombinant
Applications
IHC-P, IF/ICC, WB
Purity
Protein A affinity
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Limitations
This phospho-Histone H2AX antibody is available for research use only.
Storage Conditions
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the phospho-Histone H2AX antibody should be determined by the researcher.
Location
Nucleus
Image Legend
Frequently Asked Questions
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