Phospho-Histone H2AX Antibody (pS139)

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:1000 µg/mL, IHC (FFPE) : 1:50-1:200, IF/ICC: 1:50-1:200

UniProt

P16104

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

The amino acids surrounding a phosphorylated serine at position 139 were used as the immunogen for the phospho-Histone H2AX antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

AbH41

Type

Recombinant

Applications

IHC-P, IF/ICC, WB

Purity

Protein A affinity

Format

Purified

Buffer

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Reconstitution

After reconstitution, the phospho-Histone H2AX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This phospho-Histone H2AX antibody is available for research use only.

Storage Conditions

After reconstitution, the phospho-Histone H2AX antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the phospho-Histone H2AX antibody should be determined by the researcher.

Location

Nucleus

Image Legend

Immunofluorescent staining of human HeLa cells treated and untreated with H2O2 using phospho-Histone H2AX antibody (green) and DAPI nuclear stain (blue) .