Cytochrome C Antibody

CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow Cytometry: 1-3ug/10^6 cells, Immunofluorescence: 2-4 µg/mL

UniProt

P99999

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Human Cytochrome C recombinant protein (amino acids G2-E105) was used as the immunogen for the Cytochrome C antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

5F10

Applications

WB, IHC-P, FACS, IF

Purity

Protein G affinity

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Cytochrome C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cytochrome C antibody is available for research use only.

Storage Conditions

After reconstitution, the Cytochrome C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Cytochrome C antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Flow cytometry testing of human K562 cells with Cytochrome C antibody at 1ug/10^6 cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= Cytochrome C antibody.