HSPA2 Antibody

HSPA2 (heat shock 70kDa protein 2) is also known as HEAT-SHOCK PROTEIN, 70-KD, 2, HSP70-2, HEAT-SHOCK PROTEIN, 70-KD, 3 or HSP70-3. Analysis of the sequence indicated that HSPA2 is the human homolog of the murine Hsp70-2 gene, with 91.7% identity in the nucleotide coding sequence and 98.2% in the corresponding amino acid sequence. HSPA2 has less amino acid homology to the other members of the human HSP70 gene family. HSPA2 is constitutively expressed in most tissues, with very high levels in testis and skeletal muscle. The HSPA2 gene is located on chromosome 14q22-q24. Immunohistochemical analysis detected weak expression of HSPA2 in spermatocytes and stronger expression in spermatids and in the tail of mature sperm. HSPA2 may be critical to sperm maturation through its role as a protein chaperone.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P54652

Host

Mouse

Reactivity

Human

Immunogen

Amino acids KISEQDKNKILDKCQEVINWLDRNQMAEKDEYEHK was used as the immunogen for the HSPA2 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

4A4

Applications

WB, IHC-P

Purity

Protein G affinity

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the HSPA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HSPA2 antibody is available for research use only.

Storage Conditions

After reconstitution, the HSPA2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HSPA2 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Western blot testing of human 1) HeLa, 2) MBA-MD-231, 3) COLO-320, 4) PANC-1, 5) HT180, 6) MBA-MD-453 and 7) HepG2 lysate with HSPA2 antibody at 0.5ug/ml. Expected molecular weight ~70 kDa.