CASR Antibody / Calcium Sensing Receptor

The calcium-sensing receptor (CaSR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH) -producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P41180

Host

Mouse

Reactivity

Human

Immunogen

Human CASR recombinant protein (amino acids Q926-S1078) was used as the immunogen for the CASR antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

11E9.

Applications

Purity

Protein G affinity

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the CASR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CASR antibody is available for research use only.

Storage Conditions

After reconstitution, the CASR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CASR antibody should be determined by the researcher.

Location

Cell membrane

Image Legend

Western blot testing of human 1) HeLa, 2) A549, 3) 22RV1, 4) HepG2 and 5) Caco-2 lysate with CASR antibody at 0.5ug/ml. Predicted molecular weight ~121 kDa, but is routinely observed at up to ~160 kDa.