NOX4 Antibody / NADPH oxidase 4
Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity. [UniProt]
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:2000, Immunohistochemisty (FFPE) : 1:50-1:200, Immunofluorescence: 1:50-1:200
UniProt
Q9NPH5
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide from human NOX4 was used as the immunogen for the NOX4 antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
E-14
Type
Recombinant
Applications
WB, IHC-P, IF
Purity
Protein A affinity
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the NOX4 antibody at -20oC.
Limitations
This NOX4 antibody is available for research use only.
Storage Conditions
Store the NOX4 antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the NOX4 antibody should be determined by the researcher.
Location
Cytoplasm (ER membrane)
Image Legend
Western blot testing of human JAR cell lysate with NOX4 antibody at 0.5ug/ml. Expected molecular weight: ~65 kDa, 75-80 kDa.
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