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Smoothelin Antibody / SMSMO / SMTN

Smoothelin is a protein that in humans is encoded by the SMTN gene. This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P53814

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids QELEREEEALASKRFRAERQDNKENWLHSQQREAEQR were used as the immunogen for the Smoothelin antibody. This sequence is specific to the long form but not found in the short form of the protein.

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Smoothelin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Smoothelin antibody is available for research use only.

Storage Conditions

After reconstitution, the Smoothelin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Smoothelin antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Western blot testing of human 1) HeLa, 2) COLO-320 and 3) SW620 cell lysate with Smoothelin antibody at 0.5ug/ml. Predicted molecular weight ~99 kDa but commonly observed at ~110 kDa (long form) .

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