Ribonuclease 3 Antibody / RNASE3 / Eosinophil cationic protein

Eosinophil Cationic Protein (ECP) also known as Ribonuclease 3 is a basic protein located in the eosinophil primary matrix. In humans, the eosinophil cationic protein is encoded by the RNASE3 gene. The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. ECP is released during degranulation of eosinophils. This protein is related to inflammation and asthma because in these cases, there are increased levels of ECP in the body.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)

UniProt

P12724

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human recombinant protein (amino acids R28-I160) was used as the immunogen for the Ribonuclease 3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Ribonuclease 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Ribonuclease 3 antibody is available for research use only.

Storage Conditions

After reconstitution, the Ribonuclease 3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Ribonuclease 3 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) human Jurkat, 2) rat liver and 3) mouse liver lysate with Ribonuclease 3 antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.