Talin 1 Antibody

Talin-1 is a protein that in humans is encoded by the TLN1 gene. This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q9Y490

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids QQLAPREGISQEALHTQMLTAVQEISHLIEPLANAARAEASQ were used as the immunogen for the Talin 1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Talin 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Talin 1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Talin 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Talin 1 antibody should be determined by the researcher.

Location

Cytoplasm, Cell surface

Image Legend

Western blot testing of 1) rat lung, 2) mouse lung, 3) human U-87 MG, 4) human MDA-MB-231 and 5) human HepG2 lysate with Talin 1 antibody. Predicted molecular weight ~275 kDa.