HE4 Antibody / WFDC2

WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)

UniProt

Q14508

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human recombinant protein (amino acids E31-F124) was used as the immunogen for the HE4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the HE4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HE4 antibody is available for research use only.

Storage Conditions

After reconstitution, the HE4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HE4 antibody should be determined by the researcher.

Location

Secreted, cytoplasmic

Image Legend

Western blot testing of 1) human HeLa, 2) human MDA-MB-231, 3) human MDA-MB-451, 4) rat thymus, 5) mouse testis and 6) mouse thymus lysate with HE4 antibody at 0.5ug/ml. Predicted molecular weight: 13-25 depending on glycosylation level.