FNDC5 Antibody
Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)
UniProt
Q8NAU1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Human recombinant protein (amino acids D32-E143) was used as the immunogen for the FNDC5 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
Limitations
This FNDC5 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the FNDC5 antibody should be determined by the researcher.
Location
Cell membrane
Image Legend
Western blot testing of 1) rat heart and 2) mouse heart lysate with FNDC5 antibody at 0.5ug/ml. Predicted molecular weight ~23 kDa.
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