ANP Antibody / ANF / Atrial Natriuretic Peptide

Atrial natriuretic peptide (ANP), also known as NPPA or PND, is a powerful vasodilator, and a protein (polypeptide) hormone secreted by heart muscle cells. This gene is mapped to 1p36.22. It is involved in the homeostatic control of body water, sodium, potassium and fat (adipose tissue) . ANP is released by muscle cells in the upper chambers (atria) of the heart (atrial myocytes) in response to high blood volume. It acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. ANP has exactly the opposite function of the aldosterone secreted by the zona glomerulosa in regard to its effect on sodium in the kidney--that is, aldosterone stimulates sodium retention and it generates sodium loss.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (rat recombinant protein)

UniProt

P01161

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Rat ANP recombinant protein (amino acids N25-R122) was used as the immunogen for the ANP antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the ANP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ANP antibody is available for research use only.

Storage Conditions

After reconstitution, the ANP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ANP antibody should be determined by the researcher.

Location

Secreted

Image Legend

Western blot testing of two lots of rat heart lysate (lanes 1 & 2) and two lots of mouse heart lysate (lanes 3 & 4) with ANP antibody at 0.5ug/ml. Predicted molecular weight ~17 kDa.