ANF Antibody / ANP / Atrial Natriuretic Factor/Peptide
Atrial natriuretic factor (ANF), also known as Atrial natriuretic peptide (ANP), NPPA or PND, is a powerful vasodilator, and a protein (polypeptide) hormone secreted by heart muscle cells. This gene is mapped to 1p36.22. It is involved in the homeostatic control of body water, sodium, potassium and fat (adipose tissue) . ANF is released by muscle cells in the upper chambers (atria) of the heart (atrial myocytes) in response to high blood volume. It acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. ANF has exactly the opposite function of the aldosterone secreted by the zona glomerulosa in regard to its effect on sodium in the kidney--that is, aldosterone stimulates sodium retention and it generates sodium loss.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (recombinant mouse protein)
UniProt
P05125
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
Mouse ANP/ANF recombinant protein (amino acids N25-R122) was used as the immunogen for the ANF antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
Limitations
This ANF antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ANF antibody should be determined by the researcher.
Location
Secreted, intracellular
Image Legend
Western blot testing of two lots of mouse heart lysate with ANF antibody at 0.5ug/ml. Predicted molecular weight ~17 kDa.
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