Annexin VI Antibody

Annexin A6 (ANXA6) is a member of a family of proteins that bind membrane or cytoskeleton in a Ca (2+) -dependent manner. These proteins are characterized by homologous amino acid sequences that are present in multiple copies in each protein. ANXA6 gene is assigned to 5q32-q34 by use of a cDNA clone to probe genomic DNA from rodent-human somatic cell hybrids and for in situ hybridization. The ANX6 gene is approximately 60 kb long and contains 26 exons. The genomic sequence at the 3-prime end does not contain a canonical polyadenylylation signal. Ca (2+) -dependent binding between CRHSP28 and ANXA6 is required for acinar cell membrane trafficking events and digestive enzyme secretion.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL (recombinant human protein) (BSA-free format available)

UniProt

P08133

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A human partial recombinant protein corresponding to amino acids N395-L665 was used as the immunogen for the Annexin VI antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Annexin VI antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Annexin VI antibody is available for research use only.

Storage Conditions

After reconstitution, the Annexin VI antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Annexin VI antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human lung cancer tissue with Annexin VI antibody. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to staining.