Betacellulin Antibody / BTC

Betacellulin is a protein that in humans is encoded by the BTC gene located on chromosome 4 at locus 4q13-q21. This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted growth factor. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. This protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability. Mutations in this gene may be associated with type 2 diabetes in human patients.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (recombinant rat protein) (BSA-free format available)

UniProt

Q9JJM4

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

A rat partial recombinant protein corresponding to amino acids D32-A177 was used as the immunogen for the Betacellulin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Betacellulin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Betacellulin antibody is available for research use only.

Storage Conditions

After reconstitution, the Betacellulin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Betacellulin antibody should be determined by the researcher.

Image Legend

Western blot testing of rat 1) PC-12 and 2) RH35 lysate with Betacellulin antibody at 0.5ug/ml. Expected molecular weight: 20-40 kDa depending on glycosylation level.