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HLF Antibody / Hepatic Leukemia Factor

Hepatic leukemia factor is a protein that in humans is encoded by the HLF gene. This gene encodes a member of the proline and acidic-rich (PAR) protein family, a subset of the bZIP transcription factors. The encoded protein forms homodimers or heterodimers with other PAR family members and binds sequence-specific promoter elements to activate transcription. Chromosomal translocations fusing portions of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemias.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q16534

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids DDLKDDKYWARRRKNNMAAKRSRDARRLKE from the human protein were used as the immunogen for the HLF antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the HLF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HLF antibody is available for research use only.

Storage Conditions

After reconstitution, the HLF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HLF antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human HepG2 cells with HLF antibody (green) . HIER: steam section in pH6 citrate buffer for 20 min.

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