VEGFA Antibody

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity) . Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (recombinant mouse protein) (BSA-free format available)

UniProt

Q00731

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

A mouse partial recombinant protein corresponding to amino acids A27-N190 was used as the immunogen for the VEGFA antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the VEGFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This VEGFA antibody is available for research use only.

Storage Conditions

After reconstitution, the VEGFA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the VEGFA antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) mouse thymus, 2) mouse HEPA1-6 and 3) rat C6 cell lysate with VEGFA antibody at 0.5ug/ml. Expected molecular weight 19~22 kDa (monomer) and 38~44 kDa (dimer) . Banding may appear larger than predicted due to glycosylation.