DNA Polymerase lambda Antibody / POLL

DNA polymerase lambda, also known as POLL, is a protein found in eukaryotes. In humans, it is encoded by the POLLA gene. POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, suggesting that POLL participates in short- and long-patch base excision repair. POLL is widely expressed, with abundant expression in pachytene spermatocytes of testis and in ovary.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9UGP5

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant human protein corresponding to amino acids K307-N407 was used as the immunogen for the DNA Polymerase lambda antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the DNA Polymerase lambda antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This DNA Polymerase lambda antibody is available for research use only.

Storage Conditions

After reconstitution, the DNA Polymerase lambda antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the DNA Polymerase lambda antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of human 1) HeLa, 2) COLO-320, 3) MCF7 and 4) HepG2 cell lysate with DNA Polymerase lambda antibody at 0.5ug/ml. Predicted molecular weight ~63 kDa, also observed at ~68 kDa.