TPMT Antibody

Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT) is an enzyme that in humans is encoded by the TPMT gene. This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P51580

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids M1-K245 was used as the immunogen for the TPMT antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the TPMT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TPMT antibody is available for research use only.

Storage Conditions

After reconstitution, the TPMT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TPMT antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) human SGC-7901, 2) rat testis and 3) mouse testis lysate with TPMT antibody at 0.5ug/ml. Predicted molecular weight ~28 kDa.