CD11b Antibody / MAC-1 / ITGAM

CD11b, or Macrophage-1 alpha (MAC-1), is a glycoprotein encoded by the ITGAM gene. It has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemisty (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P11215

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids N129-E336 was used as the immunogen for the CD11b antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IHC-P, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CD11b antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CD11b antibody is available for research use only.

Storage Conditions

After reconstitution, the CD11b antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the CD11b antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, membranous

Image Legend

Flow cytometry testing of fixed human U937 cells with CD11b antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue=CD11b antibody.