EPS15 Antibody / Epidermal growth factor receptor substrate 15

Epidermal growth factor receptor substrate 15 is a protein that in humans is encoded by the EPS15 gene. This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P42566

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids A2-E88 was used as the immunogen for the EPS15 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the EPS15 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This EPS15 antibody is available for research use only.

Storage Conditions

After reconstitution, the EPS15 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the EPS15 antibody should be determined by the researcher.

Location

Cytoplasm, cell membrane

Image Legend

Western blot testing of human 1) A375, 2) HepG2, 3) Jurkat and 4) PANC-1 lysate with EPS15 antibody at 0.5ug/ml. Predicted molecular weight ~99 kDa, routinely observed at 120-150 kDa.