SCRIB Antibody / SCRIBBLE

SCRIB, also known as Scribble, SCRIBL, or Scribbled homolog (Drosophila), is a scaffold protein which in humans is encoded by the SCRIB gene. In Drosophila melanogaster, SCRIB is involved in synaptic function, neuroblast differentiation, and epithelial polarization. Mechanistically, the human homolog is a scaffold protein linked to cellular differentiation centered on the regulation of epithelial as well as neuronal morphogenesis. Deficiency in SCRIB impairs many aspects of cell polarity and cell movement. SCRIB is also likely involved in establishing apical-basal polarity as well as progression from the G1 phase to S phase in the cell cycle as a result of its relationship with cell proliferation and exocytosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, FACS: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 2-4 µg/mL

UniProt

Q14160

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant human protein corresponding to amino acids F172-K409 was used as the immunogen for the SCRIB antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IF/ICC, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the SCRIB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SCRIB antibody is available for research use only.

Storage Conditions

After reconstitution, the SCRIB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SCRIB antibody should be determined by the researcher.

Location

Cell membrane

Image Legend

Western blot testing of human 1) MCF7, 2) COLO-320, 3) 22RV1 and 4) SGC-7901 lysate with SCRIB antibody at 0.5ug/ml. Predicted molecular weight ~175 kDa, routinely observed at 200-240 kDa.