CUL4A Antibody

Cullin-4A is a protein that in humans is encoded by the CUL4A gene. It belongs to the cullin family of ubiquitin ligase proteins and is highly homologous to the CUL4B protein. CUL4A regulates numerous key processes such as DNA repair, chromatin remodeling, spermatogenesis, haematopoiesis and the mitotic cell cycle. As a result, CUL4A has been implicated in several cancers and the pathogenesis of certain viruses including HIV. Interestingly, a component of a CUL4A complex, Cereblon, was discovered to be a major target of the teratogenic agent thalidomide.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q13619

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids M494-A759 was used as the immunogen for the CUL4A antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the CUL4A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CUL4A antibody is available for research use only.

Storage Conditions

After reconstitution, the CUL4A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CUL4A antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) HeLa, 2) COLO-320, 3) MDA-MB-231, 4) Jurkat and 5) PANC-1 cell lysate with CUL4A antibody at 0.5ug/ml. Predicted molecular weight ~88 kDa.