WWOX Antibody

WW domain-containing oxidoreductase is an enzyme that in humans is encoded by the WWOX gene. This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) protein family. It spans the FRA16D common chromosomal fragile site and appears to function as a tumor suppressor gene. Expression of the encoded protein is able to induce apoptosis, while defects in this gene are associated with multiple types of cancer. Disruption of this gene is also associated with autosomal recessive spinocerebellar ataxia 12. Disruption of a similar gene in mouse results in impaired steroidogenesis, additionally suggesting a metabolic function for the protein. Alternative splicing results in multiple transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, FACS: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9NZC7

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids M1-D245 was used as the immunogen for the WWOX antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the WWOX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This WWOX antibody is available for research use only.

Storage Conditions

After reconstitution, the WWOX antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the WWOX antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Western blot testing of 1) human MCF7, 2) rat brain, 3) rat kidney, 4) rat testis, 5) mouse brain, 6) mouse kidney and 7) mouse testis lysate with WWOX antibody at 0.5ug/ml. Predicted molecular weight ~46 kDa.