MVP Antibody / Major Vault Protein

Major vault protein is a protein that in humans is encoded by the MVP gene. This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunofluorescence/Immunocytochemistry (FFPE) : 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q14764

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids A2-H259 was used as the immunogen for the MVP antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IHC-P, IF/ICC, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the MVP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MVP antibody is available for research use only.

Storage Conditions

After reconstitution, the MVP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MVP antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of human 1) placenta, 2) HepG2, 3) A549, 4) PANC-1, 5) SGC-7901 and 6) MDA-MB-231 lysate with MVP antibody at 0.5ug/ml. Observed molecular weight: 104~110 kDa.