UBA2 Antibody / SUMO-activating enzyme subunit 2 / SAE2

Ubiquitin-like 1-activating enzyme E1B (UBLE1B) also known as SUMO-activating enzyme subunit 2 (SAE2) is an enzyme that in humans is encoded by the UBA2 gene. Posttranslational modification of proteins by the addition of the small protein SUMO, or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, IF/ICC (FFPE) : 2-4 µg/mL, FACS: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9UBT2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids E449-K564 was used as the immunogen for the SAE2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/ICC, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the UBA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This UBA2 antibody is available for research use only.

Storage Conditions

After reconstitution, the UBA2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the UBA2 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of human 1) HeLa 2) placenta, 3) MCF7, 4) A549, 5) SK-OV-3, 6) 22RV1, 7) A431 and 8) COLO320 lysate with UBA2 antibody at 0.5ug/ml. Predicted molecular weight: ~72 kDa but routinely observed at ~90 kDa.

Documents

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