GLO1 Antibody / Glyoxalase I

Lactoylglutathione lyase in humans is encoded by the GLO1 gene. The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunoprecipitation: 2ug antibody/500ug lysate, Direct ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q04760

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids A2-M184 was used as the immunogen for the GLO1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IP, IF, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the GLO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This GLO1 antibody is available for research use only.

Storage Conditions

After reconstitution, the GLO1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the GLO1 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear, membrane

Image Legend

Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) COLO320, 5) 22RV1, 6) HepG2, 7) A431 and 8) U937 lysate with GLO1 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.