MRE11 Antibody / Meiotic recombination 11

Double-strand break repair protein MRE11A is a protein that in humans is encoded by the MRE11A gene. This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P49959

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids S2-D239 was used as the immunogen for the MRE11 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the MRE11 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MRE11 antibody is available for research use only.

Storage Conditions

After reconstitution, the MRE11 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MRE11 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of human 1) HeLa, 2) MCF7, 3) COLO320, 4) U-87 MG, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver lysate with MRE11 antibody at 0.5ug/ml. Predicted molecular weight ~81 kDa.