SYT1 Antibody / Synaptotagmin 1

Synaptotagmin-1 is a protein that in humans is encoded by the SYT1 gene. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca (2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells

UniProt

P21579

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids RRPIAQWHTLQVEEEVDAMLAVKK were used as the immunogen for the SYT1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, WB, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the SYT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SYT1 antibody is available for research use only.

Storage Conditions

After reconstitution, the SYT1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SYT1 antibody should be determined by the researcher.

Location

Cytoplasm, secretory vesicle membrane

Image Legend

Western blot testing of 1) rat brain and 2) mouse brain lysate with SYT1 antibody at 0.5ug/ml. Expected molecular weight: 48-65 kDa depending on glycosylation level.