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BAP1 Antibody

BAP1, also known as BRCA1-associated protein-1, contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 is a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1 is expressed as a 4-kb mRNA in all human tissues, and mapped to 3p21.3.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q92560

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant human protein corresponding to amino acids M1-E278 was used as the immunogen for the BAP1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the BAP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This BAP1 antibody is available for research use only.

Storage Conditions

After reconstitution, the BAP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the BAP1 antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) HeLa, 2) HepG2, 3) 22RV1, 4) MCF7, 5) rat thymus, 6) rat kidney and 7) mouse kidney lysate with BAP1 antibody at 0.5ug/ml. Expected molecular weight: 80-100 kDa.

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