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CD19 Antibody

B-lymphocyte antigen CD19, also known as CD19 (Cluster of Differentiation 19), is a protein that in humans is encoded by the CD19 gene. It is found on the surface of B-cells, a type of white blood cell. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. The CD19 gene encodes a cell surface molecule that assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P25918

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant mouse protein corresponding to amino acids R19-M237 was used as the immunogen for the CD19 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CD19 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CD19 antibody is available for research use only.

Storage Conditions

After reconstitution, the CD19 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CD19 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

Western blot testing of 1) human Ramos, 2) human Daudi, 3) rat spleen, 4) mouse spleen, 5) mouse RAW264.7 and 6) mouse EL4 cell lysate with CD19 antibody at 0.5ug/ml. Expected molecular weight: 60~100 kDa depending on glycosylation level.

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