RNF186 Antibody

RNF186 is an E3 ubiquitin ligase that regulates endoplasmic reticulum (ER) stress and apoptosis through its interaction with BNIP1. This gene is mapped to chromosome 1p36.13 based on an alignment of theRNF186 sequence with the genomic sequence (GRCh38) . Cotransfection and immunoprecipitation analyses showed that RNF186, through it transmembrane domains, interacted with BNIP1. Overexpression of RNF186 promoted BNIP1 ubiquitination and transfer of BNIP1 to mitochondria.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9NXI6

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant human partial protein corresponding to amino acids R86-L159 was used as the immunogen for the RNF186 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the RNF186 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This RNF186 antibody is available for research use only.

Storage Conditions

After reconstitution, the RNF186 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.