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Glycerol kinase Antibody / GK

Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis. The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD) . Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western Blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P32189

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant human partial protein corresponding to amino acids M1-S180 was used as the immunogen for the Glycerol kinase antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Glycerol kinase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Glycerol kinase antibody is available for research use only.

Storage Conditions

After reconstitution, the Glycerol kinase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Glycerol kinase antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) SW579, 2) SKOV3 and 3) U-87 MG cell lysate with Glycerol kinase antibody at 0.5ug/ml. Predicted molecular weight ~61 kDa.

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