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LIF Antibody / Leukemia inhibitory factor

LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.

Product Specifications

Specifications

Western Blot: 0.5-1 µg/mL, ELISA (Capture) : 0.1-0.5 µg/mL

UniProt

P09056

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant mouse partial protein corresponding to amino acids S24-F203 was used as the immunogen for the LIF antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the LIF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This LIF antibody is available for research use only.

Storage Conditions

After reconstitution, the LIF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Product Datasheet

https://www.nsjbio.com/tds-pdf/lif-antibody-leukemia-inhibitory-factor-rq4084

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the LIF antibody should be determined by the researcher.

CAS Number

9007-83-4

Image Legend

Western blot testing of 1) rat brain, 2) rat heart, 3) rat liver, 4) rat kidney, 5) human HeLa, 6) human placenta, 7) human Jurkat, 8) mouse liver and 9) mouse kidney lysate with LIF antibody at 0.5ug/ml. Predicted molecular weight ~23 kDa (unmodified), 32-62 kDa (glycosylated) .
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